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74. Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose nih.gov [HTML]P Kiefer, E Heinzle, O Zelder, C … - Applied and …, 2004 - Am Soc Microbiol ... A comprehensive approach to 13 C tracer studies, labeling measurements by gas chromatography-mass spectrometry, metabolite balancing, and isotopomer modeling, was applied for comparative metabolic network analysis of lysine-producing Corynebacterium glutamicum ... 70. It is all about metabolic fluxes nih.gov [HTML]J Nielsen - Journal of bacteriology, 2003 - Am Soc Microbiol http //scholar.google.com/scholar?q=related xAvLLrdbbNYJ scholar.google.com/ hl=ja as_sdt=2000 ... I. Experimental observations. Biotechnol. Bioeng. 55 305-316.[CrossRef] 21; Thykaer, J., B. Christensen, and J. Nielsen. 2002. Metabolic network analysis of an adipoyl-7-ADCA producing strain of Penicillium chrysogenum elucidation of adipate degradation.Metab. Eng. ... 47. In vivo analysis of intracellular amino acid labelings by GC/MS. C Wittmann, M Hans, E Heinzle - Analytical biochemistry, 2002 - mendeley.com ... TBDMS derivatization of amino acids is especially useful in metabolic network analysis, because M-57 fragments containing the entire carbon skeleton of the analyte can be observed by GC/MS with high signal intensities 8. The potential of the method is exemplified for ...
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metformin Metformin use and improved response to therapy in esophageal adenocarcinoma http //stemcellstm.alphamedpress.org/gca?allch= submit=Go gca=sctm%3Bsctm.2012-0058v1 Stem Cells Translational Medicine Cancer Stem Cells Glioma-Initiating Cell Elimination by Metformin Activation of FOXO3 via AMPK Atsushi Sato, Jun Sunayama, Masashi Okada, Eriko Watanabe, Shizuka Seino, Keita Shibuya, Kaori Suzuki, Yoshitaka Narita, Soichiro Shibui, Takamasa Kayama, Chifumi Kitanaka Stem Cells Trans Med first published on November 15, 2012; doi 10.5966/sctm.2012-0058 Cancer Stem Cells Glioma-Initiating Cell Elimination by Metformin Activation of FOXO3 via AMPK Control of the cancer stem/initiating cell population is considered key to realizing the long-term survival of glioblastoma patients. Recently, we demonstrated that FOXO3 activation is sufficient to induce differentiation of glioma-initiating cells having stem-like properties and inhibit their tumor-initiating potential. Here we identified metformin, an antidiabetic agent, as a therapeutic activator of FOXO3. Metformin activated FOXO3 and promoted differentiation of such stem-like glioma-initiating cells into nontumorigenic cells. Furthermore, metformin promoted FOXO3 activation and differentiation via AMP-activated protein kinase (AMPK) activation, which was sensitive to extracellular glucose availability. Importantly, transient, systemic administration of metformin depleted the self-renewing and tumor-initiating cell population within established tumors, inhibited tumor formation by stem-like glioma-initiating cells in the brain, and provided a substantial survival benefit. Our findings demonstrate that targeting glioma-initiating cells via the AMPK-FOXO3 axis is a viable therapeutic strategy against glioblastoma, with metformin being the most clinically relevant drug ever reported for targeting of glioma-initiating cells. Our results also establish a novel, direct link between glucose metabolism and cancer stem/initiating cells. Metformin May Lower Risk for Oral Cancer Development ScienceDaily (Mar. 31, 2012) http //www.sciencedaily.com/releases/2012/03/120331151138.htm http //www.news-medical.net/ http //www.news-medical.net/news/2007/08/16/28828.aspx Metformin kills some cancer cells Researchers at the University of Pennsylvania School of Medicine have found that a commonly prescribed diabetes drug kills tumor cells that lack a key regulatory gene called p53. http //www.ncbi.nlm.nih.gov/pmc/articles/PMC2756324/ Metformin Selectively Targets Cancer Stem Cells, and Acts Together with Chemotherapy to Block Tumor Growth and Prolong Remission OCT1 organic cation transporter-1 Human OCT1 is mainly expressed in the liver. LKB1 AMPK OCT2 hOCT2 - human kidney mTOR http //ds-pharma.jp/medical/ebiz/metgluco/gold_standard/012/book.pdf Polycystic Ovarian Syndrome(PCOS) http //cancerpreventionresearch.aacrjournals.org/ 乳腺腫瘍内科医のミシガン大学ポスドク留学日記 http //tkhawkeye.blog.fc2.com/blog-entry-284.html メトホルミンの作用機序は、ミトコンドリア内での酸化還元反応を抑制することでAMP→ATPへの移行を抑制する。その結果AMP優位となり、AMP-activated protein kinase（AMPK）が活性化される。その結果糖代謝の亢進やグルコースのuptakeが起こるわけだが、それと同時にmTORのカスケードを構成する因子であるRaptorやTSCを抑制することによりタンパク質の産生を抑制する。mTORはインスリンレセプターからmTOR、AKTと至るタンパク合成を促進する細胞内カスケードの中間の経路を担っているが、メトホルミンによるmTORの直接的あるいは間接的な抑制もこの経路を介した細胞増殖を抑制することにつながっている。 Biguanides suppress hepatic glucagon signalling by decreasing production of cyclic AMP Russell A. Miller, Qingwei Chu, Jianxin Xie, Marc Foretz, Benoit Viollet Morris J. Birnbaum Nature 494, 256–260 (14 February 2013) Glucose production by the liver is essential for providing a substrate for the brain during fasting. The inability of insulin to suppress hepatic glucose output is a major aetiological factor in the hyperglycaemia of type-2 diabetes mellitus and other diseases of insulin resistance1, 2. For fifty years, one of the few classes of therapeutics effective in reducing glucose production has been the biguanides, which include phenformin and metformin, the latter the most frequently prescribed drug for type-2 diabetes3. Nonetheless, the mechanism of action of biguanides remains imperfectly understood. The suggestion a decade ago that metformin reduces glucose synthesis through activation of the enzyme AMP-activated protein kinase (AMPK) has recently been challenged by genetic loss-of-function experiments4. Here we provide a novel mechanism by which metformin antagonizes the action of glucagon, thus reducing fasting glucose levels. In mouse hepatocytes, metformin leads to the accumulation of AMP and related nucleotides, which inhibit adenylate cyclase, reduce levels of cyclic AMP and protein kinase A (PKA) activity, abrogate phosphorylation of critical protein targets of PKA, and block glucagon-dependent glucose output from hepatocytes. These data support a mechanism of action for metformin involving antagonism of glucagon, and suggest an approach for the development of antidiabetic drugs. apex @ ウィキ http //www10.atwiki.jp/apex/
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She Is Set To Snag Some Golden Contracts" Would You Give Up Porn For Farming? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.221 "Japan s King Of Porn Gets His Hands Dirty Once Again -- On The Farm" Kano Sister Vanishes ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.228 "500 million yen, Foreign Lover All Part Of The Mystery Over The Disappearance Of Third Kano Sister" No Need To Hurry In A Love Hotel ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.227 " Slow sex expert says no short cut to good love hotel hanky-panky" Soapland Brothels For Women ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.223 "Frisky Femmes Frolic In Fukuoka Foam" No Cunnilingus For Japanese Women ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.225 "Why Japanese guys don t go down - here s the science " Aussie Invade Niseko ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.226 "Hokkaido Locals Want Their Own Slice Of Secret Aussie Winter Wonderland At Niseko" Can Boobs Be Too Big? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.214 "Gigantic J-cup model left lopsided after breast implosion" Bonking Buddies ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.215 "More Young People Seeing The Charms of Friends with Benefits " Forget Ping Pong Balls ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.210 "Stripper s blazing performance makes fans shout fire in a crowded theater" I m gumming! ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.201 "Hillbilly Hookers Redefine Pillow Talk" Just A Japanese Gigolo ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.204 "No Sex Please At This Rent-A-Gigolo Service" Simply Strip For Success ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.202 "Sinking Starlets Strip To Save Career" Naked Japanese Waitresses ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.205 "Japan s Mouth-Watering Way To Denude Workplace Of Sexual Harassment" Japanese School Girls Wear Skimpiest Skirts ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.203 "Shiga Prefecture s schoolgirls boast of skimpiest skirts in Japan" Japans Fetish Of Cameras And Sex ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.206 "Millions Eager To Star In Japan s Filthiest Home Videos" 151-200 Race Queens Too Racey? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.191 "Glamorous Race Queens Fess Up to Off-Track Shenanigans" Leah Dizon no Paris Hilton ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.179 "Internet leak of Personal Pics Strips Teaser of Privacy" How Big Is Your Penis? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.180 "Rising up in protest over the long and short of penis size" Japans AV Industry ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.185 "Porno world speads its legs and shoots out a few secrets" Wakatsuki Says Boobs Arent Delicious ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.162 "Cheeky Chinatsu worried sick over Japan s big breast obsession" Pensioner Porn Petering? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.159 "Healthy Dirty Mind Prevents Pensioner Porn Star from Petering Out" Kakure Kyonyu Takes Over Japan ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.181 "Japan s latest titillating trend Hiding those huge hooters!" Japan Fights For Their Rite ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.184 "Daring director uses fake groping case to take a crack at Japan s legal vultures" Clubs Clean Up ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.161 "Host clubs bond together to clean up soiled image" Cherry Poppin ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.158 "Elite Todai co-ed gets cherry popped on camera" Increase In Kiss Disease ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.157 "Increasing incidence of Kiss Disease blamed on hyper-hygienic parents" Geeks Study Hard ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.156 "Geeks can t get enough of genitalia picture book" Cum Cum Disease Strikes Japanese Women ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.153 "Deadly iku iku byo reaches a climax" 101-150 Japans Sky Rocketing STD Problem ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.139 "Crackdown On Sex Industry Drives STDs Through The Roof" Data Entry Or Flesh Flicks? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.140 "Take that job and shove it!" Miss Japans XXX Past ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.136 "Hisako Shirata Sheds Sexy Celluloid Stardom For Beauty Queen Future" Love Chairs For Couples ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.135 "Adventurous couples thrust Dream Love Chairs into overdrive" Super Model or Super Woman? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.109 "Supermodel suffers slings and arrows of outrageous fortune" Booty Camp ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.110 "Three-week boot camp whips humped-out hookers back into shape" Model Teachers ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.111 "English teaching DVDs get low down and dirty" Waiting For The Climax? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.112 "Four lusty lasses share their lives without climax" Free Love For All ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.114 "Japan s concept of Free Love lost in translation" Kansais Oldest Profession ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.113 "Kansai s red lights still glowing strong" Japan Swaps SNS for S.E.X. ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.102 "Japan s online communities turning from SNS to S.E.X." 51-100 Secret Sex Club ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.92 "Pampered pro concubines pursue a well-paid double life" Art for Porn sake ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.93 "Exposed genitalia on Japan s silver screens" Who WIll Be J-Pop Queen? ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.67 "J-Pop princesses in dirty duel for chart queen crown" Titilating Tales ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.73 "Read your gal a titillating tale" Lesbian Girlfriend? No Problem ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.72 "Sex Change Blog True or False?" 1-50 Neo-Nudism in Japan ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.50 "Stripping back to bare facts and naked truths about neo-nudism in Japan" Miss Japans Other Job ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.44 "Kurara s one little miss who doesn t miss a trick" Japans Professional Mistresses ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.37 "Life of a professional mistress not always a fistful of yen" Shibuyas Sleazy Bars ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.38 "Chaotic, erotic action at bargain prices" Natsumi Abe and the Turtle Scandal ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.36 "Turtle travail leaves Natsumi Abe fuming" Secret Slang of Tokyo Call Girls ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.32 "Just between us girls -- the secret slang splurted in the ejaculation industry" Japans Desperate Housewives ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.33 "Hungry housewives make a bit on the side through amateur porn" Rip-and-run camera crews feed the DVD market ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.24 "Panty Pulling Pandemonium Hits Japan" Secret Japanese Womens Business ｈｔｔｐ //www.asianidols.net/comment.php?comment.news.49 "For today s lewd lassies, the good vibes just keep on coming" 関連ページ 24-hour Paradise TV porn spree lets viewers get their rocks off for a better Adventurous couples thrust Dream Love Chairs into overdrive Athletic Waseda co-ed arouses school spirit (and classmates' libidos) Baby, take off your dress, but you can leave your facemask on! Campus Confidential Co-eds collect currency conducting extracurricular coitus Crackdown on sex industry drives STDs through the roof Deck the boss in cosplay folly for a good old Christmas roast English teaching DVDs get low down and dirty Hisako Shirata sheds sexy celluloid stardom for beauty queen future How to sell sex in Japan without falling foul of the 'law' Hungry housewives make a bit on the side through amateur porn Increasing incidence of 'Kiss Disease' blamed on hyper-hygienic parents Japan's online communities turning from SNS to S.E.X. Just between us girls -- the secret slang splurted in the ejaculation industry Life of a professional mistress not always a fistful of yen More gals turning backs on career jobs to feel 'wanted' turning tricks Offbeat 'panty bar' offers uninhibited lust with the improper stranger Panty sellers roll in the cash by rolling off their soiled skimpies in disabled Shiga Prefecture's schoolgirls boast of skimpiest skirts in Japan Stripping back to bare facts and naked truths about neo-nudism in Japan WaiWaiの記事を転載した英語サイト：A Why Japanese guys don't go down - here's the 'science' 毎日新聞英語版から配信された記事一覧その2 毎日新聞謝罪記事の問題点
https://w.atwiki.jp/atachi/pages/55.html
主なクラス [#n83d18cd] Webのリクエスト(WebClient版) [#vc4783ac] Webのリクエスト(HttpWebRequest) [#oceb8eb7] URLのエンコード [#dc0c93ce] .NET Frameworks Compactでのエンコード [#ydcce460] 主なクラス WebClient(MSDN) シンプルなWebアクセスを行う。ファイルのアップロードなどの機能を簡単に提供する。 HttpWebRequest(MSDN) HTTPプロコトルに特化したWebRequest HttpWebResponse(MSDN) HttpUtility(MSDN) HTTPに関するユーティリティクラスとして、System.Web名前空間に属するクラスがある。この名前空間はデフォルトでは読み込まれないので、プロジェクトの参照設定から手動で組み込む必要がある。.NETをコンパクトで実装しているプロジェクトの場合はこちら Webのリクエスト(WebClient版) WWWサーバー上のファイルを読み込むにはWebClient.OpenRead()を使うとHTTPを使用したリクエストをすべて作成してアクセスするので非常に簡単に該当のデータを読み込むことができる。 WebClient myClient = new WebClient(); Stream response = myClient.OpenRead("http //www.contoso.com/index.htm"); response.Close(); WebClientを使ったリクエストは非常にシンプルなリクエストしか行うことができない。 Webのリクエスト(HttpWebRequest) GETの場合はリクエストするURIにクエリー文字列を直接記述できる。 HttpWebRequest req = (HttpWebRequest)WebRequest.Create("http //hogehoge.com?mode=test"); WebResponse res = req.GetResponse(); Stream stream = res.GetResponseStream(); // ストリームからデータの読み込み stream.Close(); POSTの場合は、WebRequestから書き込み用のStreamを取得しそこにクエリーに含める文字列を追加する。 HttpWebRequest req = (HttpWebRequest)WebRequest.Create("http //hogehoge.com"); req.Method = "POST"; // POSTによるアクセスモード req.ContentType = "application/x-www-form-urlencoded"; // ContentTypeの指定は必須ではないが // WWWサーバーに対して行儀のよい作法。 // 相手のWWWサーバーの設定によっては、適切でないContentTypeは // リクエストを蹴られる場合があるので。 using(StreamWriter requestWriter = new StreamWriter(req.GetRequestStream())) { // C#基本構文(リソース)を参照 requestWriter.Write("mode=test"); } WebResponse res = req.GetResponse(); Stream stream = res.GetResponseStream(); // ストリームからデータの読み込み stream.Close(); 参考 using構文について URLのエンコード URLには日本語や一部の文字を含めることができません。 HTTPのリクエストでGETを使用する場合は必ずURLのエンコードが必要です。 たとえば、URLにはスペースを含めることができないので、URLのエンコードによって「%2F」というメタデータに変換されます。 URLのエンコードはHttpUtilityクラスのユーティリティメソッドを使います。 string encoded = HttpUtility.UrlEncode(target); .NET Frameworks Compactでのエンコード .NET Framework Compact で開発している場合、System.Webを参照できません。 代わりにUriクラスを使用してエンコードすることができます。(UTF-8のみ) string escaped = Uri.EscapeUriString("ほげほげ");
https://w.atwiki.jp/metalmetabo/pages/27.html
- visitors Metabolic network analysis B Christensen, J Nielsen - Bioanalysis and biosensors for bioprocess …, 2000 - Springer http //scholar.google.com/scholar?q=related PiGOMNyMhjYJ scholar.google.com/ hl=ja as_sdt=2000 Metabolic network analysis is a tool for investigating the features that identify the topology of a metabolic network and the relative activities of its individual branches. The pillars of metabolic network analysis are mathematical modeling, allowing for a quantitative analysis, ... Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast B Papp, C Pál, LD Hurst - Nature, 2004 - nature.com http //scholar.google.com/scholar?q=related 0sM60oXJ2jQJ scholar.google.com/ hl=ja as_sdt=2000 Under laboratory conditions 80% of yeast genes seem not to be essential for viability 1 . This raises the question of what the mechanistic basis for dispensability is, and whether it is the result of selection for buffering or an incidental side product. Here we analyse these issues ... Genealogy profiling through strain improvement by using metabolic network analysis metabolic flux genealogy of several generations of lysine-producing … nih.gov [HTML]C Wittmann, E Heinzle - Applied and environmental microbiology, 2002 - Am Soc Microbiol http //scholar.google.com/scholar?q=related 49I4bRT2XYIJ scholar.google.com/ hl=ja as_sdt=2000 A comprehensive approach of metabolite balancing, 13 C tracer studies, gas chromatography-mass spectrometry, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and isotopomer modeling was applied for comparative metabolic ... Metabolic network analysis of Penicillium chrysogenum using 13C-labeled glucose B Christensen, J Nielsen - Biotechnology and …, 2000 - interscience.wiley.com http //scholar.google.com/scholar?q=related 9XjgWNCKNqMJ scholar.google.com/ hl=ja as_sdt=2000 To provide a rational approach for directed genetic alter- ations in microorganisms, often referred to as metabolic engineering (Bailey, 1991; Stephanopoulos, 1999; Yarmush and Berthiaume, 1997), a detailed map of the fluxes in the central metabolism is an essential ... Metabolic network analysis of Bacillus clausii on minimal and semirich medium using 13C-labeled glucose T Christiansen, B Christensen, J Nielsen - Metabolic engineering, 2002 - Elsevier http //scholar.google.com/scholar?q=related GeX9jXLzh3EJ scholar.google.com/ hl=ja as_sdt=2000 Using 13 C-labeled glucose fed to the facultative alkalophilic Bacillus clausii producing the alkaline serine protease Savinase, the intracellular fluxes were quantified in continuous cultivation and in batch cultivation on a minimal medium. The flux through the pentose phosphate ... Genome-scale metabolic network analysis of the opportunistic pathogen Pseudomonas aeruginosa PAO1 nih.gov [HTML]MA Oberhardt, J Puchalka, KE Fryer, VAP … - Journal of …, 2008 - Am Soc Microbiol http //scholar.google.com/scholar?q=related isrhQUVHkSMJ scholar.google.com/ hl=ja as_sdt=2000 Pseudomonas aeruginosa is a major life-threatening opportunistic pathogen that commonly infects immunocompromised patients. This bacterium owes its success as a pathogen largely to its metabolic versatility and flexibility. A thorough understanding of P. aeruginosa s ... A metabolic network stoichiometry analysis of microbial growth and product formation WM Van Gulik, JJ Heijnen - Biotechnology and …, 1995 - interscience.wiley.com http //scholar.google.com/scholar?q=related p9UTGWFLJJUJ scholar.google.com/ hl=ja as_sdt=2000 ... Other work on metabolic network analysis using linear op- timization techniques has been performed on hybridoma cell Esehenchiu coli metabolism,3 .32 and acetate overflow in E. c01i.""~ Following the approach to solve underdetermined metabolic networks by conducting ... Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale C Wittmann, HM Kim, E Heinzle - Biotechnology and …, 2004 - Wiley Online Library http //scholar.google.com/scholar?q=related B7ezo5R5OxIJ scholar.google.com/ hl=ja as_sdt=2000 Abstract We present a straightforward approach com- prising 13C tracer experiments at 200-AL volume in 96-well microtiter plates with on-line measurement of dissolved oxygen for quantitative high-throughput metabolic net- work analysis at a miniaturized scale. This ... Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast biomedcentral.com [HTML]LM Blank, L Kuepfer, U Sauer - Genome Biology, 2005 - biomedcentral.com http //scholar.google.com/scholar?q=related TodhI8VNW54J scholar.google.com/ hl=ja as_sdt=2000
https://w.atwiki.jp/metalmetabo/pages/20.html
- visitors Sulfurtransferases 2010 Cell Biology of Molybdenum F Bittner, RR Mendel - Cell Biology of Metals and Nutrients, 2010 - Springer http //scholar.google.co.jp/scholar?q=related 1fBydctAk0cJ scholar.google.com/ hl=ja as_sdt=2000 Abstract The transition element molybdenum (Mo) is essential for (nearly) all biological systems as it is required by enzymes catalyzing diverse key reactions in the global carbon, sulfur,and nitrogen metabolism. The metal itself is biologically inactive unless it is complexed by a ... 2009 Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions nih.gov [HTML]A Noma, Y Sakaguchi, T Suzuki - Nucleic Acids Research, 2009 - Oxford Univ Press http //scholar.google.co.jp/scholar?q=related kS8aGPMvT5kJ scholar.google.com/ hl=ja as_sdt=2000 The wobble modification in tRNAs, 5-methoxycarbonylmethyl-2-thiouridine (mcm 5 s 2 U), is required for the proper decoding of NNR codons in eukaryotes. The 2-thio group confers conformational rigidity of mcm 5 s 2 U by largely fixing the C3 -endo ribose puckering, ensuring stable ... Origin and function of ubiquitin-like proteins nih.gov [HTML]M Hochstrasser - Nature, 2009 - nature.com http //scholar.google.co.jp/scholar?q=related vqhk3w9krT0J scholar.google.com/ hl=ja as_sdt=2000 Eukaryotic proteins can be modified through attachment to various small molecules and proteins. One such modification is conjugation to ubiquitin and ubiquitin-like proteins (UBLs), which controls an enormous range of physiological processes. Bound UBLs ... 2008 Molybdoproteomes and evolution of molybdenum utilization nih.gov [HTML]Y Zhang, VN Gladyshev - Journal of molecular biology, 2008 - Elsevier http //scholar.google.co.jp/scholar?q=related g2yHxFnhe4AJ scholar.google.com/ hl=ja as_sdt=2000 as_ylo=1981 as_yhi=2010 The trace element molybdenum (Mo) is utilized in many life forms, and it is a key component of several enzymes involved in nitrogen, sulfur, and carbon metabolism. With the exception of nitrogenase, Mo is bound in proteins to a pterin, thus forming the molybdenum cofactor ( ... Common thiolation mechanism in the biosynthesis of tRNA thiouridine and sulphur-containing cofactors nih.gov [HTML]N Shigi, Y Sakaguchi, S Asai, T Suzuki, K … - The EMBO Journal, 2008 - nature.com http //scholar.google.co.jp/scholar?q=related ARSfgx3Fp70J scholar.google.com/ hl=ja as_sdt=2000 as_ylo=2001 as_yhi=2010 2-Thioribothymidine (s 2 T), a modified uridine, is found at position 54 in transfer RNAs (tRNAs) from several thermophiles; s 2 T stabilizes the L-shaped structure of tRNA and is essential for growth at higher temperatures. Here, we identified an ATPase (tRNA-two-thiouridine ... The Sulfurtransferase Activity of Uba4 Presents a Link between Ubiquitin-like Protein Conjugation and Activation of Sulfur Carrier Proteins† J Schmitz, MM Chowdhury, P Hänzelmann, M … - Biochemistry, 2008 - ACS Publications http //scholar.google.co.jp/scholar?q=related tu5O65itnDEJ scholar.google.com/ hl=ja as_sdt=2000 as_ylo=2001 as_yhi=2010 Because of mechanistic parallels in the activation of ubiquitin and the biosynthesis of several sulfur-containing cofactors, we have characterized the human Urm1 and Saccharomyces cerevisiae Uba4 proteins, which are very similar in sequence to MOCS2A and MOCS3, respectively, ... Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems jbc.org [HTML]Y Nakai, M Nakai, H Hayashi - Journal of Biological Chemistry, 2008 - ASBMB http //scholar.google.co.jp/scholar?q=related rEPGgeDDU4AJ scholar.google.com/ hl=ja as_sdt=2000 The wobble uridine in yeast cytosolic tRNA Lys2 UUU and tRNA Glu3 UUC undergoes a thio-modification at the second position (s 2 modification) and a methoxycarbonylmethyl modification at the fifth position (mcm 5 modification). We previously demonstrated that the cytosolic ... An embarrassment of riches the enzymology of RNA modification nih.gov [HTML]D Iwata-Reuyl - Current opinion in chemical biology, 2008 - Elsevier http //scholar.google.co.jp/scholar?q=related 0tsj3spLBLEJ scholar.google.com/ hl=ja as_sdt=2000 The maturation of transfer RNA (tRNA) involves extensive chemical modification of the constituent nucleosides and results in the introduction of significant chemical diversity to tRNA. Many of the pathways to these modified nucleosides are characterized by chemically complex ... A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae cshlp.org [HTML]B Huang, J Lu, AS Byström - Rna, 2008 - rnajournal.cshlp.org http //scholar.google.co.jp/scholar?q=related 4xQVocA9SD4J scholar.google.com/ hl=ja as_sdt=2000 We recently showed that the γ-subunit of Kluyveromyces lactis killer toxin (γ-toxin) is a tRNA endonuclease that cleaves , , and 3′ of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm 5 s 2 U). The 5-methoxycarbonylmethyl ... 2007 Biosynthesis of the Thio-quinolobactin Siderophore an Interesting Variation on Sulfur Transfer nih.gov [HTML]AM Godert, M Jin, FW McLafferty, TP … - Journal of …, 2007 - Am Soc Microbiol http //scholar.google.co.jp/scholar?q=related EBTZm24E50AJ scholar.google.com/ hl=ja as_sdt=2000 Text 15 Thio-quinolobactin, 8-hydroxy-4-methoxy-thioquinaldic acid (structure 1), is a 16 ... 17400.(10-12) The genes responsible for the biosynthesis of thio-quinolobactin have 18 ... There are a number of naturally occurring molecules of diverse structure that 27 A conserved modified wobble nucleoside (mcm5s2U) in lysyl-tRNA is required for viability in yeast cshlp.org [HTML]GR Björk, B Huang, OP Persson, AS Byström - Rna, 2007 - rnajournal.cshlp.org http //scholar.google.co.jp/scholar?q=related ufP8yaCqiVsJ scholar.google.com/ hl=ja as_sdt=2000 Transfer RNAs specific for Gln, Lys, and Glu from all organisms (except Mycoplasma) and organelles have a 2-thiouridine derivative (xm 5 s 2 U) as wobble nucleoside. These tRNAs read the A- and G-ending codons in the split codon boxes His/Gln, Asn/Lys, and Asp/Glu. In ... 2006 Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain DG Waterman, M Ortiz-Lombardía, MJ Fogg, EV … - Journal of molecular …, 2006 - Elsevier http //scholar.google.co.jp/scholar?q=related j-_C2YkFA-IJ scholar.google.com/ hl=ja as_sdt=2000 as_ylo=1981 as_yhi=2010 ThiI is an enzyme responsible for the formation of the modified base S 4 U (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This base acts as a sensitive trigger for the response mechanism to UV exposure, providing protection against its damaging effects. We ... Trafficking in persulfides delivering sulfur in biosynthetic pathways EG Mueller - Nature Chemical Biology, 2006 - nature.com http //scholar.google.co.jp/scholar?q=related ZzZgR5SebvcJ scholar.google.com/ hl=ja as_sdt=2000 The presence of sulfur in cofactors has been appreciated for over a century, but the trafficking and delivery of sulfur to cofactors and nucleosides is still not fully understood. In the last decade, great strides have been made toward understanding those processes and the ... Cell biology of molybdenum RR Mendel, F Bittner - Biochimica et Biophysica Acta (BBA)-Molecular …, 2006 - Elsevier http //scholar.google.co.jp/scholar?q=related C6ckangyps8J scholar.google.com/ hl=ja as_sdt=2000 The transition element molybdenum (Mo) is of essential importance for (nearly) all biological systems as it is required by enzymes catalyzing diverse key reactions in the global carbon, sulfur and nitrogen metabolism. The metal itself is biologically inactive unless it is complexed by ... The prokaryotic antecedents of the ubiquitin-signaling system and the early evolution of ubiquitin-like β-grasp domains biomedcentral.com [HTML]LM Iyer, AM Burroughs, L Aravind - Genome Biology, 2006 - biomedcentral.com http //scholar.google.co.jp/scholar?q=related UgYKXG9Yy80J scholar.google.com/ hl=ja as_sdt=2000 Ubiquitin (Ub)-mediated signaling is one of the hallmarks of all eukaryotes. Prokaryotic homologs of Ub (ThiS and MoaD) and E1 ligases have been studied in relation to sulfur incorporation reactions in thiamine and molybdenum/tungsten cofactor biosynthesis. However, there is no ... Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions cell.com [HTML]Y Ikeuchi, N Shigi, J Kato, A Nishimura, T Suzuki - Molecular cell, 2006 - Elsevier http //scholar.google.co.jp/scholar?q=related 2Vsoqag0uDsJ scholar.google.com/ hl=ja as_sdt=2000 The wobble bases of bacterial tRNAs responsible for NNR codons are modified to 5-methylaminomethyl-2-thiouridine (mnm 5 s 2 U). 2-thio modification of mnm 5 s 2 U is required for accurate decoding and essential for normal cell growth. We identified five genes yhhP, ... 2005 Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs implications for the molecular … jbc.org [HTML]N Umeda, T Suzuki, M Yukawa, Y Ohya, H … - Journal of Biological …, 2005 - ASBMB http //scholar.google.co.jp/scholar?q=related l9eGdubEKOEJ scholar.google.com/ hl=ja as_sdt=2000 as_ylo=1981 as_yhi=2010 Human mitochondrial (mt) tRNA Lys has a taurine-containing modified uridine, 5-taurinomethyl-2-thiouridine ( m 5 s 2 U), at its anticodon wobble position. We previously found that the mt tRNA Lys , carrying the A8344G mutation from cells of patients with myoclonus ... l-Cysteine Desulfidase An [4Fe-4S] Enzyme Isolated from Methanocaldococcus jannaschii That Catalyzes the Breakdown of l-Cysteine into Pyruvate, Ammonia, and … SI Tchong, H Xu, RH White - Biochemistry, 2005 - ACS Publications http //scholar.google.co.jp/scholar?q=related QUO6D5wSaMQJ scholar.google.com/ hl=ja as_sdt=2000 as_ylo=1981 as_yhi=2010 A [4Fe-4S] enzyme that decomposes l-cysteine to hydrogen sulfide, ammonia, and pyruvate has been isolated and characterized from Methanocaldococcus jannaschii. The sequence of the isolated enzyme demonstrated that the protein was the product of the M. jannaschii ... Reconstitution of a new cysteine biosynthetic pathway in Mycobacterium tuberculosis nih.gov [HTML]KE Burns, S Baumgart, PC Dorrestein, H Zhai, … - J. Am. Chem. …, 2005 - ACS Publications http //scholar.google.co.jp/scholar?q=related 6UxhZ1K_8BAJ scholar.google.com/ hl=ja as_sdt=2000 A new pathway for cysteine biosynthesis has been elucidated in Mycobacterium tuberculosis. This pathway involves a protein-bound thiocarboxylate (CysO−SH) as the sulfide donor, similar to thiamin biosynthesis. Cysteine synthase M (CysM) catalyzes the addition ... 2004 Evidence for the physiological role of a rhodanese-like protein for the biosynthesis of the molybdenum cofactor in humans pnas.org [HTML]A Matthies, KV Rajagopalan, RR … - Proceedings of the …, 2004 - National Acad Sciences http //scholar.google.co.jp/scholar?q=related PpXEO3soBo0J scholar.google.com/ hl=ja as_sdt=2000 Recent studies have identified the human genes involved in the biosynthesis of the molybdenum cofactor. The human MOCS3 protein contains an N-terminal domain similar to the Escherichia coli MoeB protein and a C-terminal segment displaying similarities to the ... Ubiquitin-proteasome system umaryland.edu [PDF]S Fang, AM Weissman - Cellular and molecular life sciences, 2004 - Springer http //scholar.google.co.jp/scholar?q=related UwB61_LeFJAJ scholar.google.com/ hl=ja as_sdt=2000 Abstract. The capacity for exquisite regulation of ubiq- uitylation provides eukaryotic cells with a means to fine- tune both protein function and levels. This complex set of processes affects myriad proteins and potentially impacts all cellular processes. Ubiquitylation is brought about ... 2003 Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p nih.gov [HTML]AS Goehring, DM Rivers, GF Sprague Jr - Eukaryotic cell, 2003 - Am Soc Microbiol http //scholar.google.co.jp/scholar?q=related NuIwzhoOL5cJ scholar.google.com/ hl=ja as_sdt=2000 Urm1p is a ubiquitin-related protein that serves as a posttranslational modification of other proteins. Urm1p conjugation has been implicated in the budding process and in nutrient sensing. Here, we have identified the first in vivo target for the urmylation pathway as the ... 2002 The rhodanese/Cdc25 phosphatase superfamily nih.gov [HTML]D Bordo, P Bork - EMBO reports, 2002 - nature.com http //scholar.google.co.jp/scholar?q=related OxriJBcqB5AJ scholar.google.com/ hl=ja as_sdt=2000 ... In this review, the rhodanese/Cdc25 phosphatase superfamily is analyzed. ... Figure 3 Neighbor-joining tree representing the rhodanese superfamily generated by CLUSTALW (correction for ... Catalytically active rhodanese and Cdc25 phosphatase domains The N- and C- ... 2001 A sulfurtransferase is required in the transfer of cysteine sulfur in the in vitro synthesis of molybdopterin from precursor Z in Escherichia coli jbc.org [HTML]S Leimkühler, KV Rajagopalan - Journal of Biological Chemistry, 2001 - ASBMB http //scholar.google.co.jp/scholar?q=related q8ya_wsSejkJ scholar.google.com/ hl=ja as_sdt=2000 It has been shown that conversion of precursor Z to molybdopterin (MPT) by Escherichia coli MPT synthase entails the transfer of the sulfur atom of the C-terminal thiocarboxylate from the small subunit of the synthase to generate the dithiolene group of MPT and that the moeB ... M ECHANISMS U NDERLYING U BIQUITINATION uky.edu [PDF]CM Pickart - Annual review of biochemistry, 2001 - Annual Reviews http //scholar.google.co.jp/scholar?q=related YIxzrFekkFQJ scholar.google.com/ hl=ja as_sdt=2000 as_ylo=2001 as_yhi=2001 Abstract The conjugation of ubiquitin to other cellular proteins regulates a broad range of eukaryotic cell functions. The high efficiency and exquisite selectivity of ubiquitination reactions reflect the properties of enzymes known as ubiquitin-protein ligases or E3s. An E3 recognizes its ... 2000 Evolution and function of ubiquitin-like protein-conjugation systems yale.edu [PDF]M Hochstrasser - Nature cell biology, 2000 - nature.com http //scholar.google.co.jp/scholar?q=related w2aIi7-UqxoJ scholar.google.com/ hl=ja as_sdt=2000 Cellular proteins are frequently adorned with chemical modifications that alter their physical — and, as a result, their physiological — properties. Among the most spectacular of these molecular appendages are grafts of other proteins or even polymeric chains of other proteins. The ... A protein conjugation system in yeast with homology to biosynthetic enzyme reaction of prokaryotes jbc.org [HTML]K Furukawa, N Mizushima, T Noda, Y … - Journal of Biological …, 2000 - ASBMB http //scholar.google.co.jp/scholar?q=related kWwdzGB67rIJ scholar.google.com/ hl=ja as_sdt=2000 Protein conjugation, such as ubiquitination, is the process by which the C-terminal glycine of a small modifier protein is covalently attached to target protein(s) through sequential reactions with an activating enzyme and conjugating enzymes. Here we report on a novel protein ... 1998 Cysteine Sulfinate Desulfinase, a NIFS-like Protein of Escherichia coli with Selenocysteine Lyase and Cysteine Desulfurase Activities Gene Cloning, Purification and … kyoto-u.ac.jp [PDF]N Esaki, T Kurihara, T Yoshimura, K … - ICR annual …, 1998 - repository.kulib.kyoto-u.ac.jp http //scholar.google.co.jp/scholar?q=related QtLum1tHQrQJ scholar.google.com/ hl=ja as_sdt=2000 Page 1. Kyoto University KURENAI Kyoto University Research Information Repository Title Cysteine Sulfinate Desulfinase, a NIFS-like Protein of Escherichia coli with Selenocysteine Lyase and Cysteine Desulfurase Activities ... 1997 Cysteine Sulfinate Desulfinase, a NIFS-like Protein ofEscherichia coli with Selenocysteine Lyase and Cysteine Desulfurase Activities jbc.org [HTML]H Mihara, T Kurihara, T Yoshimura, K Soda, N … - Journal of Biological …, 1997 - ASBMB http //scholar.google.co.jp/scholar?q=related bOaL5q7FBDoJ scholar.google.com/ hl=ja as_sdt=2000 Selenocysteine lyase (EC 4.4.1.16) exclusively decomposes selenocysteine to alanine and elemental selenium, whereas cysteine desulfurase (NIFS protein) of Azotobacter vinelandii acts indiscriminately on both cysteine and selenocysteine to produce elemental sulfur and selenium ... 1991 Characteristics of Nicotiana tabacum nitrate reductase protein produced in Saccharomyces cerevisiae. nih.gov [PDF]HN Truong, C Meyer, F Daniel-Vedele - Biochemical Journal, 1991 - ncbi.nlm.nih.gov http //scholar.google.co.jp/scholar?q=related 8oijnWbQUp4J scholar.google.com/ hl=ja as_sdt=2000 as_ylo=1981 as_yhi=2010 INTRODUCTION Tobacco nitrate reductase (NR) (EC 1.6.6.1) is a homodimeric enzyme that reduces nitrate to nitrite in the presence of NADH (NADH NR activity). Each of its subunits contains three domains carrying respectively the prosthetic groups FAD, haem (cytochrome b-557) ... UBA 1 an essential yeast gene encoding ubiquitin-activating enzyme. nih.gov [PDF]JP McGrath, S Jentsch, A Varshavsky - The EMBO journal, 1991 - ncbi.nlm.nih.gov http //scholar.google.co.jp/scholar?q=related qFlUGfxF0asJ scholar.google.com/ hl=ja as_sdt=2000 The EMBO Journal vol.10 no.1 pp.227-236, 1991 ... UBA 1 an essential yeast gene encoding ubiquitin- ... John P.McGrath, Stefan Jentsch and Alexander Varshavsky ... Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA Present ...
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https://w.atwiki.jp/naobe/pages/78.html
ネットワークに戻る RFC2616概要 HTTP/1.1 RFC6265概要 HTTP状態管理機構 パーセントエンコーディング（URLエンコード） URLで意味を持つコードと混同する日本語などのバイナリや特殊記号を16進形式にして、先頭に%を付加した文字に変換する。正確に言うと、非予約文字、予約文字以外を変換する。 項目 説明 非予約文字 ASCIIのコード範囲内の英字（大文字・小文字）、数字、ハイフン “-”、ピリオド “.”、アンダースコア “_”、チルダ “~” 予約文字 URIを表す符号。ASCIIのコード範囲内のコロン “ ”、スラッシュ “/”、疑問符 “?”、シャープ “#”、角括弧 “[”、“]”、単価記号 “@”、感嘆符 “!”、ドル記号・ペソ記号 “$”、アンパサンド “ ”、アポストロフィー・シングルクオート “ ”、丸括弧 “(”、“)”、アスタリスク “*”、正符号・加算記号 “+”、コンマ “,”、セミコロン “;”、等号 “=” name=%E8%8F%85%E7%9B%B4%E4%BA%BA UTF-8で、%E8%8F%85：菅, %E7%9B%B4 直, %E4%BA%BA 人 x-www-form-urlencoded(URLエンコード) POSTのフォームデータをエンコードする。%を付加する方式は、パーセントエンコーディングと同じ。パーセントエンコーディングの予約文字も変換している スペースは"+"に変換。Javaのソースを見ると、英数字、"-","_", ".","*"以外を変換する BASE64 Wikipediaより データを64種類の印字可能な英数字のみを用いて、それ以外の文字を扱うことの出来ない通信環境にてマルチバイト文字やバイナリデータを扱うためのエンコード方式 7ビットのデータしか扱うことの出来ない電子メールにて広く利用されている A–Z, a–z, 0–9 までの62文字と、記号2つ (+ , /) 、さらにパディング（余った部分を詰める）のための記号として = が用いられる 76文字ごとに改行コードが入る Cookie Wikipediaより クッキーを設定する際、どの要求に対してクッキー情報を送り返すのか、URLの範囲を指定する。 規定値は、クッキーを設定したサーバに対するすべての要求であり、対象を広げることも狭めることもできる。ただし広げる場合でも、トップレベルドメインより狭い範囲でなければならない。 またクッキーの有効期限は、通常はブラウザを終了するまでだが、指定した期限まではブラウザを再度起動しても保持されるように設定することができる。有効期限の情報も、サーバからブラウザにクッキー情報を送信する段階で付加される。 無期限という設定は出来ない。 詳細： http //www.studyinghttp.net/cookies リクエスト 全てテキスト。行(CRLF)で区切られる。 フォーマット リクエストラインは１行。ヘッダは複数行。 大項目 小項目 説明 リクエストライン メソッド GET,POST,PUTなどのどれか。 リクエストURL リクエストを処理するURI プロトコルバージョン HTTPのバージョン。【例】HTTP/1.1 ヘッダ HOST リクエスト先のホスト名。必須項目 空行 POSTのときにヘッダの終了を示すために挿入される メッセージボディ POSTのときにリクエストデータを出力する 【例】 GET http //example.jp/31/31-001.php HTTP/1.1 リクエストライン Host example.jp --+ User-Agent Mozilla/5.0 (Windows NT 5.1; rv 2.0.1) Gecko/20100101 Firefox/4.0.1 | Accept text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8 +-- ヘッダ Accept-Language ja,en-us;q=0.7,en;q=0.3 | Accept-Encoding gzip, deflate | Accept-Charset Shift_JIS,utf-8;q=0.7,*;q=0.7 | Keep-Alive 115 | Connection keep-alive | Referer http //example.jp/31/ --+ Accept-CharsetでShift-JISの品質係数が１であるため、サーバは、Shift-JISでエンコードしたメッセージを返す。 POST http //example.jp/31/31-003.php HTTP/1.1 リクエストライン Accept image/gif, image/jpeg, image/pjpeg, image/pjpeg, application/vnd.ms-excel ヘッダ Referer http //example.jp/31/31-002.php Accept-Language ja User-Agent Mozilla/4.0 (compatible; MSIE 8.0; Windows NT 5.1; Trident/4.0; .NET CLR 1.1.4322; .NET CLR 2.0.50727; Inf oPath.1; .NET CLR 3.0.4506.2152; .NET CLR 3.5.30729) Content-Type application/x-www-form-urlencoded Accept-Encoding gzip, deflate Host example.jp Content-Length 65 Connection Keep-Alive Pragma no-cache name=%E8%8F%85%E7%9B%B4%E4%BA%BA mail=kan@aaa.jp gender=%E7%94%B7 メッセージボディ Hostヘッダ HTTP1.1では必須。POST/GETでのURIにHOSTを指定していなかった場合、Virtualホストを特定できない。そのためHostヘッダにHOST名を指定する。URIにホスト名があって、Hostヘッダにもホスト名がある場合はURIを優先する。URIにホスト名を指定することを強制すれば良いような気がするが。 Referer ヘッダの一種。リンク元のURLを示す。あるWebページのリンクをクリックして別のページに移動したときの、リンク元のページのこと。 改変、削除される場合があるので正しくリンクを示しているとは限らない。 セキュリティ上、問題となるのは、URLが秘密情報を含んでいる場合。たとえば、URLがセッションIDを含んでいる場合。なりすましに使われる可能性がある。 【例】 GET http //example.jp/31/31-002.php HTTP/1.1 Accept image/gif, image/jpeg, image/pjpeg, image/pjpeg, application/vnd.ms-excel, application/vnd.ms-powerpoint, appl ication/msword, application/x-shockwave-flash, application/x-ms-application, application/x-ms-xbap, application/vnd.ms -xpsdocument, application/xaml+xml, */* Referer http //example.jp/31/ Accept-Language ja User-Agent Mozilla/4.0 (compatible; MSIE 8.0; Windows NT 5.1; Trident/4.0; .NET CLR 1.1.4322; .NET CLR 2.0.50727; Inf oPath.1; .NET CLR 3.0.4506.2152; .NET CLR 3.5.30729) Accept-Encoding gzip, deflate Host example.jp Connection Keep-Alive http //example.jp/31/のHTMLのaタグからリクエストが発生したため、Refererヘッダがつく。 レスポンス 全てテキスト。行で区切られる。 フォーマット ステータスラインは１行。ヘッダ、ボディは複数行。 大項目 小項目 説明 ステータスライン プロトコルバージョン ステータスコード 1XX 処理継続、2XX 正常終了、3XX リダイレクト、4XX クライアントエラー、5XX サーバエラー テキストフレーズ レスポンスヘッダ Content-Length ボディのバイト数 Content-Type MIMEタイプ; charset=エンコーディング 空行 ヘッダの終わりを示す ボディ 【例】 HTTP/1.1 200 OK Date Wed, 04 May 2011 04 51 51 GMT Server Apache/2.2.14 (Ubuntu) X-Powered-By PHP/5.3.2-1ubuntu4.2 Vary Accept-Encoding Content-Length 20 Keep-Alive timeout=15, max=100 Connection Keep-Alive Content-Type text/html; charset=UTF-8 body 13 51 /body Content-type メディアタイプを示すエンティティヘッダ（メッセージボディに対する付随情報）。type "/" subtype *( ";" parameter )であらわされる。text/html; charset=utf-8など。charsetを追加すると出力の文字コードを示す。HTML文書にapplication/octet-streamを付けると、 多くの場合、Webブラウザはそれをダウンロードするか否か問い合わせるようになる。 Apacheの場合は、httpd.confの以下の設定でcharsetを指定する。 AddDefaultCharset UTF-8 Tomcatでは、JSPのpageディレクティブで設定する。 %@ page language="java" contentType="text/html; charset=Windows-31J" pageEncoding="Windows-31J" % http //www.atmarkit.co.jp/fjava/rensai3/mojibake01/mojibake01.html 参照
https://w.atwiki.jp/metalmetabo/pages/28.html
- visitors Application of regulatory sequence analysis and metabolic network analysis to the interpretation of gene expression data psu.edu [PDF]J Van Helden, D Gilbert, L Wernisch, M Schroeder, … - Computational …, 2001 - Springer http //scholar.google.com/scholar?q=related JS_4j3CP5nEJ scholar.google.com/ hl=ja as_sdt=2000 Comparative metabolic network analysis of two xylose fermenting recombinant Saccharomyces cerevisiae strains T Grotkjær, P Christakopoulos, J Nielsen, L … - Metabolic Engineering, 2005 - Elsevier http //scholar.google.com/scholar?q=related m6nOh8HQbaIJ scholar.google.com/ hl=ja as_sdt=2000 The recombinant xylose fermenting strain Saccharomyces cerevisiae TMB3001 can grow on xylose, but the xylose utilisation rate is low. One important reason for the inefficient fermentation of xylose to ethanol is believed to be the imbalance of redox co-factors. In the present ... The Edinburgh human metabolic network reconstruction and its functional analysis nih.gov [HTML]H Ma, A Sorokin, A Mazein, A Selkov, E … - Molecular systems …, 2007 - nature.com http //scholar.google.com/scholar?q=related pgWLepUKeS0J scholar.google.com/ hl=ja as_sdt=2000 ... A main objective of human metabolic network analysis is to see how it is related with human disease. More than 10 000 human genes (half of the whole genome) have been reported to be related with one or more human diseases in the OMIM database (Hamosh et al, 2005). ... Reconstruction of metabolic networks from genome data and analysis of their global structure for various organisms oxfordjournals.org [PDF]H Ma, AP Zeng - Bioinformatics, 2003 - Oxford Univ Press http //scholar.google.com/scholar?q=related SuHZgSLeNw8J scholar.google.com/ hl=ja as_sdt=2000 ... It is one of the main tasks of metabolic network analysis to find possible conversion pathways between two metabolites. There may be many different pathways between two metabolites, among them the shortest pathway is of particular interest for network analysis. ... Metabolic network analysis of an adipoyl-7-ADCA-producing strain of Penicillium chrysogenum elucidation of adipate degradation J Thykaer, B Christensen, J Nielsen - Metabolic Engineering, 2002 - Elsevier http //scholar.google.com/scholar?q=related gycTvJYwT9kJ scholar.google.com/ hl=ja as_sdt=2000 An adipoyl-7-ADCA-producing, recombinant strain of Penicillium chrysogenum was characterized by metabolic network analysis, with special focus on the degradation of adipate and determination of the metabolic fluxes. Degradation of the side-chain precursor, adipate, causes an ... Potential metabolic limitations in lysine production by Corynebacterium glutamicum as revealed by metabolic network analysis JA Hollander - Applied Microbiology and Biotechnology, 1994 - Springer http //scholar.google.com/scholar?q=related BwIeARCnafMJ scholar.google.com/ hl=ja as_sdt=2000 Corynebacterium glutamicum and closely related Brevi- bacterium species are the most important organisms used for the industrial fermentative production of ly- sine. Although wild-type strains do not excrete lysine at all, classical mutation programs have resulted in strains ... Metabolic flux and metabolic network analysis of Penicillium chrysogenum using 2D [13C, 1H] COSY NMR measurements and cumulative bondomer simulation WA van Winden, WM van Gulik, D … - Biotechnology and …, 2003 - Wiley Online Library http //scholar.google.com/scholar?q=related Jy6SBrqyeHAJ scholar.google.com/ hl=ja as_sdt=2000 Abstract At present two alternative methods are avail- able for analyzing the fluxes in a metabolic network (1) combining measurements of net conversion rates with a set of metabolite balances including the cofactor bal- ances, or (2) leaving out the cofactor balances and fitting the ... Metabolic‐flux and network analysis in fourteen hemiascomycetous yeasts LM Blank, F Lehmbeck, U Sauer - FEMS yeast research, 2005 - Wiley Online Library http //scholar.google.com/scholar?q=related ILl2ugZn3ZcJ scholar.google.com/ hl=ja as_sdt=2000 ... (5). Oxaloacetate14 mit was estimated using Eq. (6)[16] (6). 2.6Metabolic-network analysis. We used a previously constructed metabolic network [1,24], which consists of the PP pathway, glycolysis, TCA cycle, gluconeogenesis, malic enzyme and by-product formation reactions. ... 13C metabolic flux analysis W Wiechert - Metabolic Engineering, 2001 - Elsevier http //scholar.google.com/scholar?q=related Eat1pl-yJGQJ scholar.google.com/ hl=ja as_sdt=2000 ... 6. B. Christensen and J. Nielsen, Metabolic network analysis, a powerful tool in metabolic engineering. Adv. Biochem. Eng. ... 209–231. 7. B. Christensen and J. Nielsen, Metabolic network analysis of Penicillium chrysogenum using 13 C-labeled glucose. Biotechnol. Bioeng. ... Multiple knockout analysis of genetic robustness in the yeast metabolic network tau.ac.il [PDF]D Deutscher, I Meilijson, M Kupiec, E Ruppin - Nature genetics, 2006 - nature.com http //scholar.google.com/scholar?q=related X5cH6RUwUF0J scholar.google.com/ hl=ja as_sdt=2000 ... | PubMed |; Kitano, H. Biological robustness. Nat. Rev. Genet. 5, 826–837 (2004). | Article | PubMed | ISI | ChemPort |; Papp, B. , Pál, C. Hurst, LD Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast. Nature 429, 661–664 (2004). ...